Bismark report for: /gscratch/scrubbed/strigg/analyses/20200612/16C_32psu_2_S2_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/analyses/20200612/16C_32psu_2_S2_R2_001_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.3 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 35076228 Number of paired-end alignments with a unique best hit: 15319099 Mapping efficiency: 43.7% Sequence pairs with no alignments under any condition: 12582127 Sequence pairs did not map uniquely: 7175002 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 4095981 ((converted) top strand) GA/CT/CT: 3522702 (complementary to (converted) top strand) GA/CT/GA: 3558665 (complementary to (converted) bottom strand) CT/GA/GA: 4141751 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 539720304 Total methylated C's in CpG context: 41055352 Total methylated C's in CHG context: 964874 Total methylated C's in CHH context: 5679764 Total methylated C's in Unknown context: 110537 Total unmethylated C's in CpG context: 12540448 Total unmethylated C's in CHG context: 134466169 Total unmethylated C's in CHH context: 345013697 Total unmethylated C's in Unknown context: 429502 C methylated in CpG context: 76.6% C methylated in CHG context: 0.7% C methylated in CHH context: 1.6% C methylated in unknown context (CN or CHN): 20.5% Bismark completed in 0d 2h 4m 42s