Bismark report for: /gscratch/scrubbed/strigg/analyses/20200612/16C_26psu_3_S15_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/analyses/20200612/16C_26psu_3_S15_R2_001_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.3 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 28985030 Number of paired-end alignments with a unique best hit: 11468438 Mapping efficiency: 39.6% Sequence pairs with no alignments under any condition: 12457351 Sequence pairs did not map uniquely: 5059241 Sequence pairs which were discarded because genomic sequence could not be extracted: 1 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2761535 ((converted) top strand) GA/CT/CT: 2959301 (complementary to (converted) top strand) GA/CT/GA: 2971909 (complementary to (converted) bottom strand) CT/GA/GA: 2775692 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 425687928 Total methylated C's in CpG context: 30038233 Total methylated C's in CHG context: 839231 Total methylated C's in CHH context: 7934705 Total methylated C's in Unknown context: 72863 Total unmethylated C's in CpG context: 8350089 Total unmethylated C's in CHG context: 107681067 Total unmethylated C's in CHH context: 270844603 Total unmethylated C's in Unknown context: 354961 C methylated in CpG context: 78.2% C methylated in CHG context: 0.8% C methylated in CHH context: 2.8% C methylated in unknown context (CN or CHN): 17.0% Bismark completed in 0d 1h 47m 35s