Bismark report for: /gscratch/scrubbed/strigg/analyses/20200612/16C_26psu_2_S14_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/analyses/20200612/16C_26psu_2_S14_R2_001_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.3 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 34160543 Number of paired-end alignments with a unique best hit: 13569314 Mapping efficiency: 39.7% Sequence pairs with no alignments under any condition: 14605510 Sequence pairs did not map uniquely: 5985719 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3462159 ((converted) top strand) GA/CT/CT: 3299311 (complementary to (converted) top strand) GA/CT/GA: 3324231 (complementary to (converted) bottom strand) CT/GA/GA: 3483613 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 519211274 Total methylated C's in CpG context: 39220245 Total methylated C's in CHG context: 887081 Total methylated C's in CHH context: 4786389 Total methylated C's in Unknown context: 104894 Total unmethylated C's in CpG context: 11228640 Total unmethylated C's in CHG context: 127769258 Total unmethylated C's in CHH context: 335319661 Total unmethylated C's in Unknown context: 445945 C methylated in CpG context: 77.7% C methylated in CHG context: 0.7% C methylated in CHH context: 1.4% C methylated in unknown context (CN or CHN): 19.0% Bismark completed in 0d 2h 0m 8s