Bismark report for: 8C_32psu_3_S7_R2_001_val_2.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 100000 Number of alignments with a unique best hit from the different alignments: 51885 Mapping efficiency: 51.9% Sequences with no alignments under any condition: 22939 Sequences did not map uniquely: 25176 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 12239 ((converted) top strand) CT/GA: 12102 ((converted) bottom strand) GA/CT: 13752 (complementary to (converted) top strand) GA/GA: 13792 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 966716 Total methylated C's in CpG context: 72722 Total methylated C's in CHG context: 2317 Total methylated C's in CHH context: 19196 Total methylated C's in Unknown context: 1037 Total unmethylated C's in CpG context: 23914 Total unmethylated C's in CHG context: 234794 Total unmethylated C's in CHH context: 613773 Total unmethylated C's in Unknown context: 3663 C methylated in CpG context: 75.3% C methylated in CHG context: 1.0% C methylated in CHH context: 3.0% C methylated in Unknown context (CN or CHN): 22.1% Bismark completed in 0d 0h 1m 26s