Bismark report for: 8C_32psu_3_S7_R1_001_val_1.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 100000 Number of alignments with a unique best hit from the different alignments: 52686 Mapping efficiency: 52.7% Sequences with no alignments under any condition: 21859 Sequences did not map uniquely: 25455 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 13175 ((converted) top strand) CT/GA: 13216 ((converted) bottom strand) GA/CT: 13258 (complementary to (converted) top strand) GA/GA: 13037 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 977266 Total methylated C's in CpG context: 74108 Total methylated C's in CHG context: 2392 Total methylated C's in CHH context: 18432 Total methylated C's in Unknown context: 984 Total unmethylated C's in CpG context: 24640 Total unmethylated C's in CHG context: 239877 Total unmethylated C's in CHH context: 617817 Total unmethylated C's in Unknown context: 3745 C methylated in CpG context: 75.0% C methylated in CHG context: 1.0% C methylated in CHH context: 2.9% C methylated in Unknown context (CN or CHN): 20.8% Bismark completed in 0d 0h 0m 47s