Bismark report for: 8C_26psu_2_S10_R2_001_val_2.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 100000 Number of alignments with a unique best hit from the different alignments: 54815 Mapping efficiency: 54.8% Sequences with no alignments under any condition: 21963 Sequences did not map uniquely: 23222 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 12782 ((converted) top strand) CT/GA: 12586 ((converted) bottom strand) GA/CT: 14667 (complementary to (converted) top strand) GA/GA: 14780 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1065379 Total methylated C's in CpG context: 76825 Total methylated C's in CHG context: 2579 Total methylated C's in CHH context: 18673 Total methylated C's in Unknown context: 972 Total unmethylated C's in CpG context: 24575 Total unmethylated C's in CHG context: 256344 Total unmethylated C's in CHH context: 686383 Total unmethylated C's in Unknown context: 3815 C methylated in CpG context: 75.8% C methylated in CHG context: 1.0% C methylated in CHH context: 2.6% C methylated in Unknown context (CN or CHN): 20.3% Bismark completed in 0d 0h 1m 25s