Bismark report for: 8C_26psu_2_S10_R1_001_val_1.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 100000 Number of alignments with a unique best hit from the different alignments: 55881 Mapping efficiency: 55.9% Sequences with no alignments under any condition: 20702 Sequences did not map uniquely: 23417 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 13850 ((converted) top strand) CT/GA: 14049 ((converted) bottom strand) GA/CT: 14089 (complementary to (converted) top strand) GA/GA: 13893 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1079670 Total methylated C's in CpG context: 78390 Total methylated C's in CHG context: 2617 Total methylated C's in CHH context: 16833 Total methylated C's in Unknown context: 1012 Total unmethylated C's in CpG context: 25189 Total unmethylated C's in CHG context: 262738 Total unmethylated C's in CHH context: 693903 Total unmethylated C's in Unknown context: 3755 C methylated in CpG context: 75.7% C methylated in CHG context: 1.0% C methylated in CHH context: 2.4% C methylated in Unknown context (CN or CHN): 21.2% Bismark completed in 0d 0h 0m 47s