Bismark report for: 16C_32psu_4_S4_R2_001_val_2.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 100000 Number of alignments with a unique best hit from the different alignments: 55224 Mapping efficiency: 55.2% Sequences with no alignments under any condition: 26686 Sequences did not map uniquely: 18090 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 13287 ((converted) top strand) CT/GA: 13199 ((converted) bottom strand) GA/CT: 14351 (complementary to (converted) top strand) GA/GA: 14387 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1172484 Total methylated C's in CpG context: 77898 Total methylated C's in CHG context: 2697 Total methylated C's in CHH context: 16930 Total methylated C's in Unknown context: 1184 Total unmethylated C's in CpG context: 26108 Total unmethylated C's in CHG context: 274269 Total unmethylated C's in CHH context: 774582 Total unmethylated C's in Unknown context: 4639 C methylated in CpG context: 74.9% C methylated in CHG context: 1.0% C methylated in CHH context: 2.1% C methylated in Unknown context (CN or CHN): 20.3% Bismark completed in 0d 0h 1m 28s