Bismark report for: 16C_32psu_4_S4_R1_001_val_1.fq.gz and 16C_32psu_4_S4_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 49269 Mapping efficiency: 49.3% Sequence pairs with no alignments under any condition: 35632 Sequence pairs did not map uniquely: 15099 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 12146 ((converted) top strand) GA/CT/CT: 12511 (complementary to (converted) top strand) GA/CT/GA: 12351 (complementary to (converted) bottom strand) CT/GA/GA: 12261 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2041112 Total methylated C's in CpG context: 138394 Total methylated C's in CHG context: 4493 Total methylated C's in CHH context: 27719 Total methylated C's in Unknown context: 1693 Total unmethylated C's in CpG context: 44636 Total unmethylated C's in CHG context: 484479 Total unmethylated C's in CHH context: 1341391 Total unmethylated C's in Unknown context: 6242 C methylated in CpG context: 75.6% C methylated in CHG context: 0.9% C methylated in CHH context: 2.0% C methylated in unknown context (CN or CHN): 21.3% Bismark completed in 0d 0h 0m 36s