Bismark report for: 16C_26psu_1_S13_R2_001_val_2.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 100000 Number of alignments with a unique best hit from the different alignments: 52081 Mapping efficiency: 52.1% Sequences with no alignments under any condition: 25661 Sequences did not map uniquely: 22258 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 12090 ((converted) top strand) CT/GA: 12132 ((converted) bottom strand) GA/CT: 13879 (complementary to (converted) top strand) GA/GA: 13980 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1039579 Total methylated C's in CpG context: 76228 Total methylated C's in CHG context: 2512 Total methylated C's in CHH context: 18276 Total methylated C's in Unknown context: 1151 Total unmethylated C's in CpG context: 25308 Total unmethylated C's in CHG context: 249054 Total unmethylated C's in CHH context: 668201 Total unmethylated C's in Unknown context: 4232 C methylated in CpG context: 75.1% C methylated in CHG context: 1.0% C methylated in CHH context: 2.7% C methylated in Unknown context (CN or CHN): 21.4% Bismark completed in 0d 0h 1m 26s