Bismark report for: CTRL_8C_26psu_1_S17_R1_001_val_1.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.5 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 100000 Number of alignments with a unique best hit from the different alignments: 55056 Mapping efficiency: 55.1% Sequences with no alignments under any condition: 23710 Sequences did not map uniquely: 21234 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 13535 ((converted) top strand) CT/GA: 13098 ((converted) bottom strand) GA/CT: 14081 (complementary to (converted) top strand) GA/GA: 14342 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1082195 Total methylated C's in CpG context: 73587 Total methylated C's in CHG context: 2118 Total methylated C's in CHH context: 12403 Total methylated C's in Unknown context: 606 Total unmethylated C's in CpG context: 23043 Total unmethylated C's in CHG context: 251193 Total unmethylated C's in CHH context: 719851 Total unmethylated C's in Unknown context: 2698 C methylated in CpG context: 76.2% C methylated in CHG context: 0.8% C methylated in CHH context: 1.7% C methylated in Unknown context (CN or CHN): 18.3% Bismark completed in 0d 0h 0m 47s