Bismark report for: 8C_26psu_2_S10_R2_001_val_2.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.5 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 100000 Number of alignments with a unique best hit from the different alignments: 52459 Mapping efficiency: 52.5% Sequences with no alignments under any condition: 24778 Sequences did not map uniquely: 22763 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 12192 ((converted) top strand) CT/GA: 12055 ((converted) bottom strand) GA/CT: 14010 (complementary to (converted) top strand) GA/GA: 14202 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1015357 Total methylated C's in CpG context: 74013 Total methylated C's in CHG context: 2261 Total methylated C's in CHH context: 14814 Total methylated C's in Unknown context: 658 Total unmethylated C's in CpG context: 23008 Total unmethylated C's in CHG context: 245717 Total unmethylated C's in CHH context: 655544 Total unmethylated C's in Unknown context: 2556 C methylated in CpG context: 76.3% C methylated in CHG context: 0.9% C methylated in CHH context: 2.2% C methylated in Unknown context (CN or CHN): 20.5% Bismark completed in 0d 0h 1m 26s