Bismark report for: 8C_26psu_2_S10_R1_001_val_1.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.5 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 100000 Number of alignments with a unique best hit from the different alignments: 53663 Mapping efficiency: 53.7% Sequences with no alignments under any condition: 23425 Sequences did not map uniquely: 22912 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 13263 ((converted) top strand) CT/GA: 13514 ((converted) bottom strand) GA/CT: 13553 (complementary to (converted) top strand) GA/GA: 13333 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1034830 Total methylated C's in CpG context: 75638 Total methylated C's in CHG context: 2303 Total methylated C's in CHH context: 14137 Total methylated C's in Unknown context: 684 Total unmethylated C's in CpG context: 23725 Total unmethylated C's in CHG context: 252393 Total unmethylated C's in CHH context: 666634 Total unmethylated C's in Unknown context: 2627 C methylated in CpG context: 76.1% C methylated in CHG context: 0.9% C methylated in CHH context: 2.1% C methylated in Unknown context (CN or CHN): 20.7% Bismark completed in 0d 0h 0m 47s