Bismark report for: 8C_26psu_2_S10_R1_001_val_1.fq.gz and 8C_26psu_2_S10_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.5 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 48413 Mapping efficiency: 48.4% Sequence pairs with no alignments under any condition: 31106 Sequence pairs did not map uniquely: 20481 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 12494 ((converted) top strand) GA/CT/CT: 11755 (complementary to (converted) top strand) GA/CT/GA: 11518 (complementary to (converted) bottom strand) CT/GA/GA: 12646 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1820121 Total methylated C's in CpG context: 134838 Total methylated C's in CHG context: 3819 Total methylated C's in CHH context: 21511 Total methylated C's in Unknown context: 992 Total unmethylated C's in CpG context: 41434 Total unmethylated C's in CHG context: 448107 Total unmethylated C's in CHH context: 1170412 Total unmethylated C's in Unknown context: 3769 C methylated in CpG context: 76.5% C methylated in CHG context: 0.8% C methylated in CHH context: 1.8% C methylated in unknown context (CN or CHN): 20.8% Bismark completed in 0d 0h 0m 36s