Bismark report for: 16C_32psu_4_S4_R2_001_val_2.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.5 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 100000 Number of alignments with a unique best hit from the different alignments: 52522 Mapping efficiency: 52.5% Sequences with no alignments under any condition: 29928 Sequences did not map uniquely: 17550 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 12594 ((converted) top strand) CT/GA: 12512 ((converted) bottom strand) GA/CT: 13658 (complementary to (converted) top strand) GA/GA: 13758 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1113857 Total methylated C's in CpG context: 74566 Total methylated C's in CHG context: 2348 Total methylated C's in CHH context: 13546 Total methylated C's in Unknown context: 796 Total unmethylated C's in CpG context: 24304 Total unmethylated C's in CHG context: 261579 Total unmethylated C's in CHH context: 737514 Total unmethylated C's in Unknown context: 3137 C methylated in CpG context: 75.4% C methylated in CHG context: 0.9% C methylated in CHH context: 1.8% C methylated in Unknown context (CN or CHN): 20.2% Bismark completed in 0d 0h 1m 32s