Bismark report for: CTRL_8C_26psu_1_S17_R2_001_val_2.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.3 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 100000 Number of alignments with a unique best hit from the different alignments: 48213 Mapping efficiency: 48.2% Sequences with no alignments under any condition: 32054 Sequences did not map uniquely: 19733 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 11172 ((converted) top strand) CT/GA: 11258 ((converted) bottom strand) GA/CT: 13003 (complementary to (converted) top strand) GA/GA: 12780 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 943100 Total methylated C's in CpG context: 64531 Total methylated C's in CHG context: 1567 Total methylated C's in CHH context: 8348 Total methylated C's in Unknown context: 216 Total unmethylated C's in CpG context: 19672 Total unmethylated C's in CHG context: 217465 Total unmethylated C's in CHH context: 631517 Total unmethylated C's in Unknown context: 959 C methylated in CpG context: 76.6% C methylated in CHG context: 0.7% C methylated in CHH context: 1.3% C methylated in Unknown context (CN or CHN): 18.4% Bismark completed in 0d 0h 1m 25s