Bismark report for: CTRL_8C_26psu_1_S17_R1_001_val_1.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.3 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 100000 Number of alignments with a unique best hit from the different alignments: 49920 Mapping efficiency: 49.9% Sequences with no alignments under any condition: 30255 Sequences did not map uniquely: 19825 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 12184 ((converted) top strand) CT/GA: 11855 ((converted) bottom strand) GA/CT: 12843 (complementary to (converted) top strand) GA/GA: 13038 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 976683 Total methylated C's in CpG context: 67473 Total methylated C's in CHG context: 1568 Total methylated C's in CHH context: 7868 Total methylated C's in Unknown context: 199 Total unmethylated C's in CpG context: 20155 Total unmethylated C's in CHG context: 226608 Total unmethylated C's in CHH context: 653011 Total unmethylated C's in Unknown context: 1008 C methylated in CpG context: 77.0% C methylated in CHG context: 0.7% C methylated in CHH context: 1.2% C methylated in Unknown context (CN or CHN): 16.5% Bismark completed in 0d 0h 0m 49s