Bismark report for: 8C_32psu_3_S7_R2_001_val_2.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.3 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 100000 Number of alignments with a unique best hit from the different alignments: 44367 Mapping efficiency: 44.4% Sequences with no alignments under any condition: 32568 Sequences did not map uniquely: 23065 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 10402 ((converted) top strand) CT/GA: 10333 ((converted) bottom strand) GA/CT: 11816 (complementary to (converted) top strand) GA/GA: 11816 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 812651 Total methylated C's in CpG context: 63079 Total methylated C's in CHG context: 1289 Total methylated C's in CHH context: 8427 Total methylated C's in Unknown context: 191 Total unmethylated C's in CpG context: 19142 Total unmethylated C's in CHG context: 201035 Total unmethylated C's in CHH context: 519679 Total unmethylated C's in Unknown context: 885 C methylated in CpG context: 76.7% C methylated in CHG context: 0.6% C methylated in CHH context: 1.6% C methylated in Unknown context (CN or CHN): 17.8% Bismark completed in 0d 0h 1m 25s