Bismark report for: 16C_32psu_4_S4_R2_001_val_2.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.3 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 100000 Number of alignments with a unique best hit from the different alignments: 46428 Mapping efficiency: 46.4% Sequences with no alignments under any condition: 37473 Sequences did not map uniquely: 16099 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 10964 ((converted) top strand) CT/GA: 10894 ((converted) bottom strand) GA/CT: 12228 (complementary to (converted) top strand) GA/GA: 12342 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 979597 Total methylated C's in CpG context: 66722 Total methylated C's in CHG context: 1703 Total methylated C's in CHH context: 8037 Total methylated C's in Unknown context: 262 Total unmethylated C's in CpG context: 20800 Total unmethylated C's in CHG context: 231180 Total unmethylated C's in CHH context: 651155 Total unmethylated C's in Unknown context: 1108 C methylated in CpG context: 76.2% C methylated in CHG context: 0.7% C methylated in CHH context: 1.2% C methylated in Unknown context (CN or CHN): 19.1% Bismark completed in 0d 0h 1m 28s