Bismark report for: 16C_32psu_4_S4_R1_001_val_1.fq.gz and 16C_32psu_4_S4_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.3 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 40669 Mapping efficiency: 40.7% Sequence pairs with no alignments under any condition: 45723 Sequence pairs did not map uniquely: 13608 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 10049 ((converted) top strand) GA/CT/CT: 10236 (complementary to (converted) top strand) GA/CT/GA: 10104 (complementary to (converted) bottom strand) CT/GA/GA: 10280 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1671819 Total methylated C's in CpG context: 115871 Total methylated C's in CHG context: 2870 Total methylated C's in CHH context: 13994 Total methylated C's in Unknown context: 362 Total unmethylated C's in CpG context: 35330 Total unmethylated C's in CHG context: 397248 Total unmethylated C's in CHH context: 1106506 Total unmethylated C's in Unknown context: 1520 C methylated in CpG context: 76.6% C methylated in CHG context: 0.7% C methylated in CHH context: 1.2% C methylated in unknown context (CN or CHN): 19.2% Bismark completed in 0d 0h 0m 35s