Bismark report for: 16C_26psu_1_S13_R2_001_val_2.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.3 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 100000 Number of alignments with a unique best hit from the different alignments: 43770 Mapping efficiency: 43.8% Sequences with no alignments under any condition: 36268 Sequences did not map uniquely: 19962 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 10057 ((converted) top strand) CT/GA: 10139 ((converted) bottom strand) GA/CT: 11733 (complementary to (converted) top strand) GA/GA: 11841 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 862501 Total methylated C's in CpG context: 64796 Total methylated C's in CHG context: 1458 Total methylated C's in CHH context: 8096 Total methylated C's in Unknown context: 219 Total unmethylated C's in CpG context: 20019 Total unmethylated C's in CHG context: 209898 Total unmethylated C's in CHH context: 558234 Total unmethylated C's in Unknown context: 948 C methylated in CpG context: 76.4% C methylated in CHG context: 0.7% C methylated in CHH context: 1.4% C methylated in Unknown context (CN or CHN): 18.8% Bismark completed in 0d 0h 1m 29s