Bismark report for: CTRL_8C_26psu_1_S17_R1_001_val_1.fq.gz and CTRL_8C_26psu_1_S17_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 39628 Mapping efficiency: 39.6% Sequence pairs with no alignments under any condition: 44283 Sequence pairs did not map uniquely: 16089 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 10213 ((converted) top strand) GA/CT/CT: 9698 (complementary to (converted) top strand) GA/CT/GA: 9694 (complementary to (converted) bottom strand) CT/GA/GA: 10023 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1490664 Total methylated C's in CpG context: 105847 Total methylated C's in CHG context: 2114 Total methylated C's in CHH context: 9210 Total methylated C's in Unknown context: 96 Total unmethylated C's in CpG context: 30451 Total unmethylated C's in CHG context: 346371 Total unmethylated C's in CHH context: 996671 Total unmethylated C's in Unknown context: 695 C methylated in CpG context: 77.7% C methylated in CHG context: 0.6% C methylated in CHH context: 0.9% C methylated in unknown context (CN or CHN): 12.1% Bismark completed in 0d 0h 0m 34s