Bismark report for: 8C_32psu_3_S7_R1_001_val_1.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 100000 Number of alignments with a unique best hit from the different alignments: 42014 Mapping efficiency: 42.0% Sequences with no alignments under any condition: 36014 Sequences did not map uniquely: 21972 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 10534 ((converted) top strand) CT/GA: 10547 ((converted) bottom strand) GA/CT: 10530 (complementary to (converted) top strand) GA/GA: 10403 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 765495 Total methylated C's in CpG context: 59723 Total methylated C's in CHG context: 1155 Total methylated C's in CHH context: 6663 Total methylated C's in Unknown context: 66 Total unmethylated C's in CpG context: 17981 Total unmethylated C's in CHG context: 190257 Total unmethylated C's in CHH context: 489716 Total unmethylated C's in Unknown context: 417 C methylated in CpG context: 76.9% C methylated in CHG context: 0.6% C methylated in CHH context: 1.3% C methylated in Unknown context (CN or CHN): 13.7% Bismark completed in 0d 0h 0m 47s