Bismark report for: 8C_26psu_2_S10_R1_001_val_1.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 100000 Number of alignments with a unique best hit from the different alignments: 45236 Mapping efficiency: 45.2% Sequences with no alignments under any condition: 34460 Sequences did not map uniquely: 20304 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 11105 ((converted) top strand) CT/GA: 11396 ((converted) bottom strand) GA/CT: 11423 (complementary to (converted) top strand) GA/GA: 11312 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 863224 Total methylated C's in CpG context: 64658 Total methylated C's in CHG context: 1378 Total methylated C's in CHH context: 6186 Total methylated C's in Unknown context: 68 Total unmethylated C's in CpG context: 18949 Total unmethylated C's in CHG context: 210976 Total unmethylated C's in CHH context: 561077 Total unmethylated C's in Unknown context: 476 C methylated in CpG context: 77.3% C methylated in CHG context: 0.6% C methylated in CHH context: 1.1% C methylated in Unknown context (CN or CHN): 12.5% Bismark completed in 0d 0h 0m 48s