Bismark report for: 16C_26psu_1_S13_R1_001_val_1.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 100000 Number of alignments with a unique best hit from the different alignments: 41443 Mapping efficiency: 41.4% Sequences with no alignments under any condition: 39661 Sequences did not map uniquely: 18896 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 10122 ((converted) top strand) CT/GA: 10203 ((converted) bottom strand) GA/CT: 10507 (complementary to (converted) top strand) GA/GA: 10611 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 811452 Total methylated C's in CpG context: 61638 Total methylated C's in CHG context: 1250 Total methylated C's in CHH context: 6314 Total methylated C's in Unknown context: 81 Total unmethylated C's in CpG context: 18623 Total unmethylated C's in CHG context: 198495 Total unmethylated C's in CHH context: 525132 Total unmethylated C's in Unknown context: 459 C methylated in CpG context: 76.8% C methylated in CHG context: 0.6% C methylated in CHH context: 1.2% C methylated in Unknown context (CN or CHN): 15.0% Bismark completed in 0d 0h 0m 49s