Bismark report for: /gscratch/scrubbed/strigg/analyses/20200427/TG_PE_FASTQS/cat_lanes/Sealice_F1_S20_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/analyses/20200427/TG_PE_FASTQS/cat_lanes/Sealice_F1_S20_R2_001_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Caligus/GENOMES/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 224907116 Number of paired-end alignments with a unique best hit: 62174415 Mapping efficiency: 27.6% Sequence pairs with no alignments under any condition: 112764706 Sequence pairs did not map uniquely: 49967995 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 17584459 ((converted) top strand) GA/CT/CT: 13556057 (complementary to (converted) top strand) GA/CT/GA: 13538432 (complementary to (converted) bottom strand) CT/GA/GA: 17495467 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2340133640 Total methylated C's in CpG context: 4105081 Total methylated C's in CHG context: 3385525 Total methylated C's in CHH context: 21614155 Total methylated C's in Unknown context: 1617188 Total unmethylated C's in CpG context: 354444893 Total unmethylated C's in CHG context: 366501181 Total unmethylated C's in CHH context: 1590082805 Total unmethylated C's in Unknown context: 21333560 C methylated in CpG context: 1.1% C methylated in CHG context: 0.9% C methylated in CHH context: 1.3% C methylated in unknown context (CN or CHN): 7.0% Bismark completed in 0d 8h 42m 44s