Bismark report for: /gscratch/scrubbed/strigg/analyses/20200422/TG_SE_FASTQS/16C_26psu_4_S16_L002_R1_001_trimmed.fq.gz (version: v0.22.3) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 17053995 Number of alignments with a unique best hit from the different alignments: 8994207 Mapping efficiency: 52.7% Sequences with no alignments under any condition: 4092460 Sequences did not map uniquely: 3967328 Sequences which were discarded because genomic sequence could not be extracted: 1 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 2120189 ((converted) top strand) CT/GA: 2123356 ((converted) bottom strand) GA/CT: 2372580 (complementary to (converted) top strand) GA/GA: 2378081 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 171266072 Total methylated C's in CpG context: 12629378 Total methylated C's in CHG context: 441967 Total methylated C's in CHH context: 3593105 Total methylated C's in Unknown context: 176525 Total unmethylated C's in CpG context: 3969643 Total unmethylated C's in CHG context: 41682252 Total unmethylated C's in CHH context: 108949727 Total unmethylated C's in Unknown context: 644852 C methylated in CpG context: 76.1% C methylated in CHG context: 1.0% C methylated in CHH context: 3.2% C methylated in Unknown context (CN or CHN): 21.5% Bismark completed in 0d 0h 32m 28s