Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/CTRL_16C_26psu_2_S21_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/CTRL_16C_26psu_2_S21_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 26786269 Number of paired-end alignments with a unique best hit: 12858057 Mapping efficiency: 48.0% Sequence pairs with no alignments under any condition: 9690132 Sequence pairs did not map uniquely: 4238080 Sequence pairs which were discarded because genomic sequence could not be extracted: 5 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3417874 ((converted) top strand) GA/CT/CT: 2969413 (complementary to (converted) top strand) GA/CT/GA: 3007443 (complementary to (converted) bottom strand) CT/GA/GA: 3463322 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 513006152 Total methylated C's in CpG context: 45390037 Total methylated C's in CHG context: 2243211 Total methylated C's in CHH context: 8974383 Total methylated C's in Unknown context: 979368 Total unmethylated C's in CpG context: 13353405 Total unmethylated C's in CHG context: 131475715 Total unmethylated C's in CHH context: 311569401 Total unmethylated C's in Unknown context: 2112980 C methylated in CpG context: 77.3% C methylated in CHG context: 1.7% C methylated in CHH context: 2.8% C methylated in unknown context (CN or CHN): 31.7% Bismark completed in 0d 2h 32m 37s