Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/8C_32psu_2_S6_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/8C_32psu_2_S6_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 25763427 Number of paired-end alignments with a unique best hit: 12952124 Mapping efficiency: 50.3% Sequence pairs with no alignments under any condition: 9084939 Sequence pairs did not map uniquely: 3726364 Sequence pairs which were discarded because genomic sequence could not be extracted: 6 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3272941 ((converted) top strand) GA/CT/CT: 3177554 (complementary to (converted) top strand) GA/CT/GA: 3202466 (complementary to (converted) bottom strand) CT/GA/GA: 3299157 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 551458860 Total methylated C's in CpG context: 41782829 Total methylated C's in CHG context: 1632387 Total methylated C's in CHH context: 8351461 Total methylated C's in Unknown context: 693554 Total unmethylated C's in CpG context: 13640310 Total unmethylated C's in CHG context: 138067668 Total unmethylated C's in CHH context: 347984205 Total unmethylated C's in Unknown context: 1833359 C methylated in CpG context: 75.4% C methylated in CHG context: 1.2% C methylated in CHH context: 2.3% C methylated in unknown context (CN or CHN): 27.4% Bismark completed in 0d 2h 40m 56s