Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/8C_26psu_3_S11_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/8C_26psu_3_S11_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 34321341 Number of paired-end alignments with a unique best hit: 17826817 Mapping efficiency: 51.9% Sequence pairs with no alignments under any condition: 10476173 Sequence pairs did not map uniquely: 6018351 Sequence pairs which were discarded because genomic sequence could not be extracted: 4 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 4624932 ((converted) top strand) GA/CT/CT: 4245017 (complementary to (converted) top strand) GA/CT/GA: 4285424 (complementary to (converted) bottom strand) CT/GA/GA: 4671440 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 699554745 Total methylated C's in CpG context: 57458459 Total methylated C's in CHG context: 2409324 Total methylated C's in CHH context: 12365811 Total methylated C's in Unknown context: 1016891 Total unmethylated C's in CpG context: 17438274 Total unmethylated C's in CHG context: 180082888 Total unmethylated C's in CHH context: 429799989 Total unmethylated C's in Unknown context: 2334206 C methylated in CpG context: 76.7% C methylated in CHG context: 1.3% C methylated in CHH context: 2.8% C methylated in unknown context (CN or CHN): 30.3% Bismark completed in 0d 3h 31m 42s