Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_32psu_1_S1_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_32psu_1_S1_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 39102702 Number of paired-end alignments with a unique best hit: 19691598 Mapping efficiency: 50.4% Sequence pairs with no alignments under any condition: 12987429 Sequence pairs did not map uniquely: 6423675 Sequence pairs which were discarded because genomic sequence could not be extracted: 8 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 5118401 ((converted) top strand) GA/CT/CT: 4691306 (complementary to (converted) top strand) GA/CT/GA: 4722488 (complementary to (converted) bottom strand) CT/GA/GA: 5159395 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 806679054 Total methylated C's in CpG context: 62217457 Total methylated C's in CHG context: 2615560 Total methylated C's in CHH context: 13352468 Total methylated C's in Unknown context: 1101328 Total unmethylated C's in CpG context: 19375443 Total unmethylated C's in CHG context: 200621423 Total unmethylated C's in CHH context: 508496703 Total unmethylated C's in Unknown context: 2697527 C methylated in CpG context: 76.3% C methylated in CHG context: 1.3% C methylated in CHH context: 2.6% C methylated in unknown context (CN or CHN): 29.0% Bismark completed in 0d 3h 58m 48s