Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_26psu_1_S13_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_26psu_1_S13_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/chr1-29MT/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 44133837 Number of paired-end alignments with a unique best hit: 21575808 Mapping efficiency: 48.9% Sequence pairs with no alignments under any condition: 16272400 Sequence pairs did not map uniquely: 6285629 Sequence pairs which were discarded because genomic sequence could not be extracted: 6 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 5540754 ((converted) top strand) GA/CT/CT: 5203089 (complementary to (converted) top strand) GA/CT/GA: 5247188 (complementary to (converted) bottom strand) CT/GA/GA: 5584771 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 942816382 Total methylated C's in CpG context: 70456126 Total methylated C's in CHG context: 2900590 Total methylated C's in CHH context: 14160894 Total methylated C's in Unknown context: 1242988 Total unmethylated C's in CpG context: 23036552 Total unmethylated C's in CHG context: 234618921 Total unmethylated C's in CHH context: 597643299 Total unmethylated C's in Unknown context: 3242207 C methylated in CpG context: 75.4% C methylated in CHG context: 1.2% C methylated in CHH context: 2.3% C methylated in unknown context (CN or CHN): 27.7% Bismark completed in 0d 4h 35m 25s