Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/CTRL_16C_26psu_2_S21_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/CTRL_16C_26psu_2_S21_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 26786269 Number of paired-end alignments with a unique best hit: 12679248 Mapping efficiency: 47.3% Sequence pairs with no alignments under any condition: 9163307 Sequence pairs did not map uniquely: 4943714 Sequence pairs which were discarded because genomic sequence could not be extracted: 1471 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3372005 ((converted) top strand) GA/CT/CT: 2929703 (complementary to (converted) top strand) GA/CT/GA: 2962926 (complementary to (converted) bottom strand) CT/GA/GA: 3413143 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 505786145 Total methylated C's in CpG context: 44889169 Total methylated C's in CHG context: 2207015 Total methylated C's in CHH context: 8797220 Total methylated C's in Unknown context: 967404 Total unmethylated C's in CpG context: 12901262 Total unmethylated C's in CHG context: 129880538 Total unmethylated C's in CHH context: 307110941 Total unmethylated C's in Unknown context: 2059405 C methylated in CpG context: 77.7% C methylated in CHG context: 1.7% C methylated in CHH context: 2.8% C methylated in unknown context (CN or CHN): 32.0% Bismark completed in 0d 2h 48m 45s