Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/8C_32psu_4_S8_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/8C_32psu_4_S8_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 29264212 Number of paired-end alignments with a unique best hit: 14084982 Mapping efficiency: 48.1% Sequence pairs with no alignments under any condition: 10598154 Sequence pairs did not map uniquely: 4581076 Sequence pairs which were discarded because genomic sequence could not be extracted: 1429 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3506326 ((converted) top strand) GA/CT/CT: 3510550 (complementary to (converted) top strand) GA/CT/GA: 3536275 (complementary to (converted) bottom strand) CT/GA/GA: 3530402 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 653044712 Total methylated C's in CpG context: 47026493 Total methylated C's in CHG context: 1940321 Total methylated C's in CHH context: 8179862 Total methylated C's in Unknown context: 980538 Total unmethylated C's in CpG context: 15830901 Total unmethylated C's in CHG context: 157094385 Total unmethylated C's in CHH context: 422972750 Total unmethylated C's in Unknown context: 2443521 C methylated in CpG context: 74.8% C methylated in CHG context: 1.2% C methylated in CHH context: 1.9% C methylated in unknown context (CN or CHN): 28.6% Bismark completed in 0d 3h 21m 38s