Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/8C_32psu_1_S5_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/8C_32psu_1_S5_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 27906731 Number of paired-end alignments with a unique best hit: 14072066 Mapping efficiency: 50.4% Sequence pairs with no alignments under any condition: 7509546 Sequence pairs did not map uniquely: 6325119 Sequence pairs which were discarded because genomic sequence could not be extracted: 1403 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3624722 ((converted) top strand) GA/CT/CT: 3379551 (complementary to (converted) top strand) GA/CT/GA: 3407536 (complementary to (converted) bottom strand) CT/GA/GA: 3658854 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 524069701 Total methylated C's in CpG context: 44580153 Total methylated C's in CHG context: 1794653 Total methylated C's in CHH context: 9364823 Total methylated C's in Unknown context: 713305 Total unmethylated C's in CpG context: 13859065 Total unmethylated C's in CHG context: 136364840 Total unmethylated C's in CHH context: 318106167 Total unmethylated C's in Unknown context: 1648842 C methylated in CpG context: 76.3% C methylated in CHG context: 1.3% C methylated in CHH context: 2.9% C methylated in unknown context (CN or CHN): 30.2% Bismark completed in 0d 3h 2m 25s