Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_32psu_4_S4_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_32psu_4_S4_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 31260279 Number of paired-end alignments with a unique best hit: 15436486 Mapping efficiency: 49.4% Sequence pairs with no alignments under any condition: 11416596 Sequence pairs did not map uniquely: 4407197 Sequence pairs which were discarded because genomic sequence could not be extracted: 1571 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3828514 ((converted) top strand) GA/CT/CT: 3869966 (complementary to (converted) top strand) GA/CT/GA: 3890343 (complementary to (converted) bottom strand) CT/GA/GA: 3846092 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 721445725 Total methylated C's in CpG context: 49301552 Total methylated C's in CHG context: 2005888 Total methylated C's in CHH context: 9694813 Total methylated C's in Unknown context: 886134 Total unmethylated C's in CpG context: 15534385 Total unmethylated C's in CHG context: 173483751 Total unmethylated C's in CHH context: 471425336 Total unmethylated C's in Unknown context: 2315476 C methylated in CpG context: 76.0% C methylated in CHG context: 1.1% C methylated in CHH context: 2.0% C methylated in unknown context (CN or CHN): 27.7% Bismark completed in 0d 3h 41m 41s