Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_32psu_3_S3_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_32psu_3_S3_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 27293279 Number of paired-end alignments with a unique best hit: 14077908 Mapping efficiency: 51.6% Sequence pairs with no alignments under any condition: 7639032 Sequence pairs did not map uniquely: 5576339 Sequence pairs which were discarded because genomic sequence could not be extracted: 1641 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3758456 ((converted) top strand) GA/CT/CT: 3263750 (complementary to (converted) top strand) GA/CT/GA: 3278875 (complementary to (converted) bottom strand) CT/GA/GA: 3775186 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 562213851 Total methylated C's in CpG context: 42389529 Total methylated C's in CHG context: 1897697 Total methylated C's in CHH context: 10650157 Total methylated C's in Unknown context: 790890 Total unmethylated C's in CpG context: 13177716 Total unmethylated C's in CHG context: 143348150 Total unmethylated C's in CHH context: 350750602 Total unmethylated C's in Unknown context: 1719039 C methylated in CpG context: 76.3% C methylated in CHG context: 1.3% C methylated in CHH context: 2.9% C methylated in unknown context (CN or CHN): 31.5% Bismark completed in 0d 3h 8m 57s