Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_32psu_2_S2_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_32psu_2_S2_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 35355537 Number of paired-end alignments with a unique best hit: 18964065 Mapping efficiency: 53.6% Sequence pairs with no alignments under any condition: 9596391 Sequence pairs did not map uniquely: 6795081 Sequence pairs which were discarded because genomic sequence could not be extracted: 1968 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 5040279 ((converted) top strand) GA/CT/CT: 4389671 (complementary to (converted) top strand) GA/CT/GA: 4434918 (complementary to (converted) bottom strand) CT/GA/GA: 5097229 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 770362160 Total methylated C's in CpG context: 56862554 Total methylated C's in CHG context: 2552593 Total methylated C's in CHH context: 14987501 Total methylated C's in Unknown context: 1096199 Total unmethylated C's in CpG context: 18225219 Total unmethylated C's in CHG context: 193626816 Total unmethylated C's in CHH context: 484107477 Total unmethylated C's in Unknown context: 2307573 C methylated in CpG context: 75.7% C methylated in CHG context: 1.3% C methylated in CHH context: 3.0% C methylated in unknown context (CN or CHN): 32.2% Bismark completed in 0d 4h 10m 45s