Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_32psu_1_S1_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_32psu_1_S1_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 39102702 Number of paired-end alignments with a unique best hit: 19406595 Mapping efficiency: 49.6% Sequence pairs with no alignments under any condition: 12183912 Sequence pairs did not map uniquely: 7512195 Sequence pairs which were discarded because genomic sequence could not be extracted: 2059 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 5046317 ((converted) top strand) GA/CT/CT: 4624992 (complementary to (converted) top strand) GA/CT/GA: 4649906 (complementary to (converted) bottom strand) CT/GA/GA: 5083321 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 795043989 Total methylated C's in CpG context: 61507148 Total methylated C's in CHG context: 2567479 Total methylated C's in CHH context: 13095077 Total methylated C's in Unknown context: 1086181 Total unmethylated C's in CpG context: 18699831 Total unmethylated C's in CHG context: 198109732 Total unmethylated C's in CHH context: 501064722 Total unmethylated C's in Unknown context: 2607755 C methylated in CpG context: 76.7% C methylated in CHG context: 1.3% C methylated in CHH context: 2.5% C methylated in unknown context (CN or CHN): 29.4% Bismark completed in 0d 4h 26m 40s