Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_26psu_4_S16_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_26psu_4_S16_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 34400085 Number of paired-end alignments with a unique best hit: 16901015 Mapping efficiency: 49.1% Sequence pairs with no alignments under any condition: 11211337 Sequence pairs did not map uniquely: 6287733 Sequence pairs which were discarded because genomic sequence could not be extracted: 1935 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 4147379 ((converted) top strand) GA/CT/CT: 4280174 (complementary to (converted) top strand) GA/CT/GA: 4297075 (complementary to (converted) bottom strand) CT/GA/GA: 4174452 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 706890716 Total methylated C's in CpG context: 53200529 Total methylated C's in CHG context: 2089496 Total methylated C's in CHH context: 12724965 Total methylated C's in Unknown context: 814187 Total unmethylated C's in CpG context: 15958390 Total unmethylated C's in CHG context: 177822938 Total unmethylated C's in CHH context: 445094398 Total unmethylated C's in Unknown context: 2179425 C methylated in CpG context: 76.9% C methylated in CHG context: 1.2% C methylated in CHH context: 2.8% C methylated in unknown context (CN or CHN): 27.2% Bismark completed in 0d 4h 3m 9s