Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_26psu_2_S14_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_26psu_2_S14_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 34354958 Number of paired-end alignments with a unique best hit: 16975867 Mapping efficiency: 49.4% Sequence pairs with no alignments under any condition: 11400230 Sequence pairs did not map uniquely: 5978861 Sequence pairs which were discarded because genomic sequence could not be extracted: 1964 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 4316125 ((converted) top strand) GA/CT/CT: 4145375 (complementary to (converted) top strand) GA/CT/GA: 4174149 (complementary to (converted) bottom strand) CT/GA/GA: 4338254 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 743440841 Total methylated C's in CpG context: 54903499 Total methylated C's in CHG context: 2218723 Total methylated C's in CHH context: 12206626 Total methylated C's in Unknown context: 961446 Total unmethylated C's in CpG context: 16575990 Total unmethylated C's in CHG context: 185279605 Total unmethylated C's in CHH context: 472256398 Total unmethylated C's in Unknown context: 2380618 C methylated in CpG context: 76.8% C methylated in CHG context: 1.2% C methylated in CHH context: 2.5% C methylated in unknown context (CN or CHN): 28.8% Bismark completed in 0d 4h 3m 27s