Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_26psu_1_S13_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_26psu_1_S13_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 44133837 Number of paired-end alignments with a unique best hit: 21245424 Mapping efficiency: 48.1% Sequence pairs with no alignments under any condition: 15237259 Sequence pairs did not map uniquely: 7651154 Sequence pairs which were discarded because genomic sequence could not be extracted: 2533 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 5461006 ((converted) top strand) GA/CT/CT: 5123633 (complementary to (converted) top strand) GA/CT/GA: 5163286 (complementary to (converted) bottom strand) CT/GA/GA: 5494966 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 929088288 Total methylated C's in CpG context: 69696287 Total methylated C's in CHG context: 2836903 Total methylated C's in CHH context: 13848735 Total methylated C's in Unknown context: 1228682 Total unmethylated C's in CpG context: 22222326 Total unmethylated C's in CHG context: 231470893 Total unmethylated C's in CHH context: 589013144 Total unmethylated C's in Unknown context: 3128931 C methylated in CpG context: 75.8% C methylated in CHG context: 1.2% C methylated in CHH context: 2.3% C methylated in unknown context (CN or CHN): 28.2% Bismark completed in 0d 5h 5m 7s