Output will be written into the directory: /gscratch/scrubbed/strigg/analyses/20190814_Calig/ Processing paired-end Bismark output file(s) (SAM format): /gscratch/scrubbed/strigg/analyses/20190814_Calig/Sealice_F1_S20_R1_001_val_1_bismark_bt2_pe.bam /gscratch/scrubbed/strigg/analyses/20190814_Calig/Sealice_F2_S22_R1_001_val_1_bismark_bt2_pe.bam If there are several alignments to a single position in the genome the first alignment will be chosen. Since the input files are not in any way sorted this is a near-enough random selection of reads. Checking file >>/gscratch/scrubbed/strigg/analyses/20190814_Calig/Sealice_F1_S20_R1_001_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: Sealice_F1_S20_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in /gscratch/scrubbed/strigg/analyses/20190814_Calig/Sealice_F1_S20_R1_001_val_1_bismark_bt2_pe.bam: 63327608 Total number duplicated alignments removed: 23521989 (37.14%) Duplicated alignments were found at: 12771810 different position(s) Total count of deduplicated leftover sequences: 39805619 (62.86% of total) Checking file >>/gscratch/scrubbed/strigg/analyses/20190814_Calig/Sealice_F2_S22_R1_001_val_1_bismark_bt2_pe.bam<< for signs of file truncation... ...passed! Output file is: Sealice_F2_S22_R1_001_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in /gscratch/scrubbed/strigg/analyses/20190814_Calig/Sealice_F2_S22_R1_001_val_1_bismark_bt2_pe.bam: 35458887 Total number duplicated alignments removed: 8834707 (24.92%) Duplicated alignments were found at: 5486581 different position(s) Total count of deduplicated leftover sequences: 26624180 (75.08% of total)