Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/Sealice_F1_S20_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/Sealice_F1_S20_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Caligus/GENOMES/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 225746922 Number of paired-end alignments with a unique best hit: 63327611 Mapping efficiency: 28.1% Sequence pairs with no alignments under any condition: 121134921 Sequence pairs did not map uniquely: 41284390 Sequence pairs which were discarded because genomic sequence could not be extracted: 3 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 17963269 ((converted) top strand) GA/CT/CT: 13634042 (complementary to (converted) top strand) GA/CT/GA: 13707963 (complementary to (converted) bottom strand) CT/GA/GA: 18022334 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2717362145 Total methylated C's in CpG context: 5831950 Total methylated C's in CHG context: 5234399 Total methylated C's in CHH context: 28580018 Total methylated C's in Unknown context: 3139344 Total unmethylated C's in CpG context: 414300782 Total unmethylated C's in CHG context: 433425327 Total unmethylated C's in CHH context: 1829989669 Total unmethylated C's in Unknown context: 24817838 C methylated in CpG context: 1.4% C methylated in CHG context: 1.2% C methylated in CHH context: 1.5% C methylated in unknown context (CN or CHN): 11.2% Bismark completed in 0d 17h 9m 40s