Bismark report for: /gscratch/srlab/strigg/data/Ssalar/FASTQS/RAW/16C_26psu_1_S13_L001_R1_001.fastq.gz and /gscratch/srlab/strigg/data/Ssalar/FASTQS/RAW/16C_26psu_1_S13_L001_R2_001.fastq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 22721 Mapping efficiency: 22.7% Sequence pairs with no alignments under any condition: 72870 Sequence pairs did not map uniquely: 4409 Sequence pairs which were discarded because genomic sequence could not be extracted: 5 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 5782 ((converted) top strand) GA/CT/CT: 5481 (complementary to (converted) top strand) GA/CT/GA: 5599 (complementary to (converted) bottom strand) CT/GA/GA: 5854 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1331795 Total methylated C's in CpG context: 99771 Total methylated C's in CHG context: 8690 Total methylated C's in CHH context: 25724 Total methylated C's in Unknown context: 5813 Total unmethylated C's in CpG context: 31936 Total unmethylated C's in CHG context: 326811 Total unmethylated C's in CHH context: 838863 Total unmethylated C's in Unknown context: 7810 C methylated in CpG context: 75.8% C methylated in CHG context: 2.6% C methylated in CHH context: 3.0% C methylated in unknown context (CN or CHN): 42.7% Bismark completed in 0d 0h 1m 43s