Bismark report for: /gscratch/scrubbed/strigg/analyses/20190806_100K_Salmo_test5bp/16C_26psu_1_S13_L001_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/analyses/20190806_100K_Salmo_test5bp/16C_26psu_1_S13_L001_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 48633 Mapping efficiency: 48.6% Sequence pairs with no alignments under any condition: 34055 Sequence pairs did not map uniquely: 17312 Sequence pairs which were discarded because genomic sequence could not be extracted: 9 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 12521 ((converted) top strand) GA/CT/CT: 11738 (complementary to (converted) top strand) GA/CT/GA: 11785 (complementary to (converted) bottom strand) CT/GA/GA: 12580 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2128840 Total methylated C's in CpG context: 160096 Total methylated C's in CHG context: 6381 Total methylated C's in CHH context: 30862 Total methylated C's in Unknown context: 2753 Total unmethylated C's in CpG context: 50751 Total unmethylated C's in CHG context: 531950 Total unmethylated C's in CHH context: 1348800 Total unmethylated C's in Unknown context: 7073 C methylated in CpG context: 75.9% C methylated in CHG context: 1.2% C methylated in CHH context: 2.2% C methylated in unknown context (CN or CHN): 28.0% Bismark completed in 0d 0h 1m 26s