Bismark report for: /gscratch/srlab/strigg/data/Salmo_Calig/TRIM_1st5bp/16C_26psu_1_S13_L001_R1_001_trimmed.5bp_3prime.fq.gz and /gscratch/srlab/strigg/data/Salmo_Calig/TRIM_1st5bp/16C_26psu_1_S13_L001_R2_001_trimmed.5bp_3prime.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 529 Mapping efficiency: 0.5% Sequence pairs with no alignments under any condition: 99269 Sequence pairs did not map uniquely: 202 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 140 ((converted) top strand) GA/CT/CT: 124 (complementary to (converted) top strand) GA/CT/GA: 126 (complementary to (converted) bottom strand) CT/GA/GA: 139 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 23240 Total methylated C's in CpG context: 1641 Total methylated C's in CHG context: 92 Total methylated C's in CHH context: 420 Total methylated C's in Unknown context: 30 Total unmethylated C's in CpG context: 621 Total unmethylated C's in CHG context: 5927 Total unmethylated C's in CHH context: 14539 Total unmethylated C's in Unknown context: 85 C methylated in CpG context: 72.5% C methylated in CHG context: 1.5% C methylated in CHH context: 2.8% C methylated in unknown context (CN or CHN): 26.1% Bismark completed in 0d 0h 1m 16s