Bismark report for: /gscratch/scrubbed/strigg/analyses/20190806_100K_Salmo_test5bp/AS-0.6/16C_26psu_1_S13_L001_R2_001_val_2.fq.gz_unmapped_reads_2.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 51367 Number of alignments with a unique best hit from the different alignments: 7113 Mapping efficiency: 13.8% Sequences with no alignments under any condition: 26265 Sequences did not map uniquely: 17989 Sequences which were discarded because genomic sequence could not be extracted: 1 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 1281 ((converted) top strand) CT/GA: 1207 ((converted) bottom strand) GA/CT: 2267 (complementary to (converted) top strand) GA/GA: 2357 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 189153 Total methylated C's in CpG context: 12213 Total methylated C's in CHG context: 670 Total methylated C's in CHH context: 6516 Total methylated C's in Unknown context: 482 Total unmethylated C's in CpG context: 4733 Total unmethylated C's in CHG context: 41746 Total unmethylated C's in CHH context: 123275 Total unmethylated C's in Unknown context: 1985 C methylated in CpG context: 72.1% C methylated in CHG context: 1.6% C methylated in CHH context: 5.0% C methylated in Unknown context (CN or CHN): 19.5% Bismark completed in 0d 0h 0m 59s