Bismark report for: /gscratch/scrubbed/strigg/analyses/20190806_100K_Salmo_test5bp/AS-0.6/16C_26psu_1_S13_L001_R1_001_val_1.fq.gz_unmapped_reads_1.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 51367 Number of alignments with a unique best hit from the different alignments: 8232 Mapping efficiency: 16.0% Sequences with no alignments under any condition: 25002 Sequences did not map uniquely: 18133 Sequences which were discarded because genomic sequence could not be extracted: 1 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 1580 ((converted) top strand) CT/GA: 1623 ((converted) bottom strand) GA/CT: 2540 (complementary to (converted) top strand) GA/GA: 2488 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 206837 Total methylated C's in CpG context: 13983 Total methylated C's in CHG context: 814 Total methylated C's in CHH context: 6580 Total methylated C's in Unknown context: 530 Total unmethylated C's in CpG context: 5530 Total unmethylated C's in CHG context: 48529 Total unmethylated C's in CHH context: 131401 Total unmethylated C's in Unknown context: 2102 C methylated in CpG context: 71.7% C methylated in CHG context: 1.6% C methylated in CHH context: 4.8% C methylated in Unknown context (CN or CHN): 20.1% Bismark completed in 0d 0h 0m 53s