Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/CTRL_16C_26psu_2_S21_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/CTRL_16C_26psu_2_S21_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/ with the specified options: -q --score-min L,0,-2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 76588 Mapping efficiency: 76.6% Sequence pairs with no alignments under any condition: 119 Sequence pairs did not map uniquely: 23293 Sequence pairs which were discarded because genomic sequence could not be extracted: 11 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 20406 ((converted) top strand) GA/CT/CT: 17798 (complementary to (converted) top strand) GA/CT/GA: 17954 (complementary to (converted) bottom strand) CT/GA/GA: 20419 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 3116592 Total methylated C's in CpG context: 249629 Total methylated C's in CHG context: 24032 Total methylated C's in CHH context: 148027 Total methylated C's in Unknown context: 25877 Total unmethylated C's in CpG context: 97811 Total unmethylated C's in CHG context: 738471 Total unmethylated C's in CHH context: 1858622 Total unmethylated C's in Unknown context: 75950 C methylated in CpG context: 71.8% C methylated in CHG context: 3.2% C methylated in CHH context: 7.4% C methylated in unknown context (CN or CHN): 25.4% Bismark completed in 0d 0h 2m 30s